The former Reading youngster had to be substituted late on in his side's 2-0 defeat to Cardiff on Saturday following an aerial challenge with Leon Barnett.
Scans later revealed a groin strain that has instantly ruled the 22-year-old out for the rest of the campaign.
It represents a significant setback for the Owls, who are currently slumped down in 21st place in the Championship, just a point above the drop zone.
Antonio has been a key player for the Yorkshire club this season, hitting nine goals and supplying a further nine assists in 42 appearances.
"He'll be back for pre-season and that's the target," said boss Dave Jones. "It's happened, it's nobody's fault and we just have to make sure that everything we do is the right thing and we get him fully fit for the start of pre-season."
Jones also hinted that he may seek to bring a replacement in on loan, adding: "We're looking at the loan market and have been keeping an eye on that all along and we will see if there is someone we can bring in.
"If there isn't, then we will go with what we've got. It's no good getting someone in to bolster the squad, it's got to be someone who will give us an advantage."