The defender was left out of their 2-1 victory over promotion-chasing Scunthorpe on Saturday due to a hip problem but will return to the matchday squad.
Midfielder Ryan Ledson misses out as he is away on international duty with the England Under-20 squad.
Appleton could make changes to his starting line-up as the U’s prepare for four games in 13 days, including the Checkatrade Trophy final at Wembley on April 2.
Jay Spearing could be back in contention to start for Bolton.
The former Liverpool midfielder returned after three games out with a knee injury as a late substitute in Saturday’s victory over Northampton.
It had initially been feared Spearing could miss the rest of the season but he underlined his speedy return to fitness by playing a part in Filipe Morais’ winning goal.
The Trotters have no new injury or suspension concerns as they look to extend the three-game winning run that has pushed them into the automatic promotion places.
England boss Gareth Southgate believes Jack Wilshere will be fit enough to feature in Arsenal's clash against Stoke City on Sunday.
Celtic forward Scott Sinclair has reportedly emerged as a summer transfer target for Premier League outfit Bournemouth.
Thibaut Courtois says he will still be at Chelsea next year, even if he does not agree a new contract this summer.
Stoke City defender Ryan Shawcross is believed to be a summer transfer target for new Southampton boss Mark Hughes.