Arsenal loanee Afobe represented Bolton in the competition during last month’s loan spell with the club, while Woolford is also ineligible having played for former club Bristol City in the third round earlier this year.
Striker Andy Keogh, who came on for Afobe in the closing stages of last weekend’s 2-1 defeat to Blackpool, is in contention to replace him in the starting line-up, with Liam Feeney ready to step in for Woolford.
On-loan Derby forward Nathan Tyson remains sidelined with ankle ligament damage, while injured midfielder Chris Taylor is no closer to making a return to first-team action.
A goal apiece from James Tomkins and Luka Milivojevic handed Crystal Palace a crucial 2-0 win over Huddersfield at the John Smith's Stadium on Saturday evening.
Manchester United bounced back from their Champions League exit as they beat Brighton 2-0 at Old Trafford on Saturday night to reach the FA Cup semi-finals.
Tottenham reached the FA Cup semi-finals after they cruised to a 3-0 victory over Swansea in their quarter-final clash at the Liberty Stadium on Saturday.
Premier League basement club West Brom squandered the lead as they suffered a 2-1 defeat to Bournemouth at the Vitality Stadium on Saturday.
Chesterfield boss Jack Lester felt his side were unlucky not to claim all three points after their 1-1 draw at Cheltenham.
New Swindon manager Phil Brown revelled in his side's victory at Cambridge in his first game in charge.