A 3-1 setback on home soil at the hands of the Championship strugglers means Boro have now gone two games without a win - ironically since going top of the English second tier with victory over Sheffield Wednesday earlier this month.
Back-to-back losses have eased expectation levels on Teesside but Mowbray has said he cannot afford passengers and will not tolerate a repeat performance.
On Tuesday they take on Simon Grayson's Huddersfield Town at the Riverside and Mowbray has said he is hoping to see a response from his players.
"We need to get back to winning ways as quickly as we can," said the Middlesbrough manager. "If you're on a winning streak, you feel as though you can pick any 11 players and send them out to win the game."
He added: "We'll try and pick a team for this game to get us back to winning ways. We've just come off a decent winning run and the momentum that's behind you helps no matter what you have to do with the team."