However, goalkeeper Tommy Lee will miss the Spireites' final game of the season through injury.
Lester is out of contract in the summer and will not be offered a new deal. The veteran striker, 37, has missed the last two matches with a back complaint but he will play on Saturday and lead the team out.
Other changes to the starting line-up will be necessary as Lee suffered an ankle injury in training this week and Richard Brindley has a hernia problem.
Full-back Brindley was forced off during the first half against Fleetwood last weekend, when Chesterfield's play-off hopes were extinguished despite victory at Highbury.
Richard O'Donnell will be called back into the squad due to Lee's injury while defender Sam Hird, midfielders Alex Henshall, Danny Whitaker and Craig Clay and strikers Scott Boden and Armand Gnanduillet are available for recalls and teenage forward Jonathan Wafula could be included on the bench.