Midfielders David Syers and Paul Keegan and striker Iain Hume all returned from injury against Yeovil on Saturday without suffering any ill effects.
Keegan played the full 90 minutes while Syers and Hume were introduced from the bench midway through the second half of the 1-1 draw with the Glovers.
Long-term absentees Chris Brown and Andy Griffin aside, Martin Woods is now the only player unavailable through injury. The midfielder will be out for around a month after suffering a tear in a calf muscle.
On-loan Everton midfielder John Lundstram made his Doncaster, and senior football, debut from the start at the weekend while Cameron Howieson was an unused substitute following his loan switch from Burnley.
Howieson, Syers, Hume, winger Kyle Bennett and on-loan striker Emile Sinclair are options for Flynn should he wish to make changes in search of Rovers' first league win in six matches.