Luke Summerfield, Charles Vernam, Paul Dixon and Karleigh Osborne are all expected to miss the game, with Summerfield and Dixon having started against Carlisle last week and Vernam coming on as a substitute.
In addition, Simeon Jackson and Mallik Wilks are unlikely to return from hamstring problems, leaving Jolley with reduced options for a crucial game.
The two sides, who sit in 20th and 22nd place in the table respectively, are just a point apart and too close for comfort to the relegation zone.
Port Vale boss Neil Aspin will be without striker Tom Pope as he recovers from hernia surgery.
The club’s 16-goal top scorer, who has been playing through the pain since before Christmas, is likely to miss several games as the season enters a crucial phase.
Marcus Harness is among several players Aspin could look towards to plug a sizeable gap as the Valiants attempt to drag themselves further clear of the bottom two.
Defender Antony Kay has returned to light training after also undergoing hernia surgery, although he is still some way away from match fitness.
Southampton manager Mark Hughes was delighted to see his new side reach the FA Cup semi-finals but also warned that the Premier League remains a concern.
Borussia Dortmund winger Andre Schurrle was delighted with the team's hard-fought 1-0 Bundesliga victory over Hannover 96 on Sunday afternoon.
Chelsea defender Andreas Christensen has revealed that he sought advice from club legend John Terry after making a few costly mistakes recently.