Skipper Duffy and fellow defender Tootle were both sidelined through injury as County’s unbeaten home record was ended by Exeter on Saturday.
January arrivals Liam Noble, Matty Virtue and Noor Husin will be hoping to keep their places in the starting line-up.
Shola Ameobi is pushing for a start after two substitute appearances since returning from injury.
Crawley welcome back midfielder Josh Payne from suspension.
The former West Ham trainee has completed a three-match ban after he was sent off against Stevenage last month.
Dannie Bulman will be assessed after he missed Friday night’s 2-1 defeat at Newport with a knee problem.
Watford boss Javi Gracia does not think Abdoulaye Doucoure's form will be affected by his omission from France's latest squad.