Rovers eased a nervy start with clinical first-half goals from Adam Armstrong and Elliott Bennett.
However, a Michael Jacobs’ volley and Max Power’s free-kick in the space of 10 second-half minutes earned Wigan a point.
The hosts remain top, six points clear of Wigan, but the visitors have four games in hand over their Lancashire rivals and reasserted their title credentials.
Blackburn hit the front in the sixth minute as Bradley Dack’s through-ball was hammered into the bottom corner by Armstrong – his fifth goal for Rovers.
That lead was doubled 11 minutes later. Dack was again the architect and Bennett slammed home his second goal of the week from close range.
Corry Evans’ volley was tipped behind as Blackburn sought to finish the game off, but Wigan fought back and reduced the deficit in the 63rd minute.
A cross caused confusion in the box and Jacobs took advantage to volley home from 12 yards, with theball appearing to take a slight deflection off team-mate Will Grigg on its way in.
The comeback was completed 17 minutes from time when Power’s set-piece from the left touchline evaded everybody and went in, sending the visiting supporters into raptures.
Paul Huntington says Preston's play-off hopes this season hinge on them getting the balance right on and off the pitch.