Wenger often treats the competition as a way to blood youngsters in the first team so the likes of Ainsley Maitland-Niles, Jeff Reine-Adelaide and Rob Holding will hope to be involved.
Third-choice goalkeeper Emiliano Martinez is expected to start while Lucas Perez is in contention following an ankle injury but Santi Cazorla (Achilles), Mathieu Debuchy (hamstring), Hector Bellerin (ankle), Per Mertesacker and Danny Welbeck (both knee) are all out.
Provisional squad: Cech, Ospina, Martinez, Jenkinson, Holding, Gabriel, Koscielny, Mustafi, Gibbs, Monreal, Maitland-Niles, Elneny, Coquelin, Ramsey, Xhaka, Oxlade-Chamberlain, Zelalem, Ozil, Walcott, Reine-Adelaide, Iwobi, Sanchez, Akpom, Perez, Giroud, Willock.
Colchester will hand late fitness tests to Ryan Jackson and Brandon Comley prior to their clash with Yeovil.
Arsenal secured their place in the quarter-finals of the Europa League following their 3-1 defeat of AC Milan at the Emirates Stadium on Thursday.
Danny Welbeck scored twice against AC Milan to toast his England recall by sending Arsenal into the last eight of the Europa League.
Ipswich striker Joe Garner will not play again this season after it emerged he suffered a fractured skull against Sheffield United last week.