Captain Gary Cahill was rested for the midweek Carabao Cup semi-final, first-leg draw with Arsenal and could return in defence.
Ross Barkley is expected to be fit to make his debut following a hamstring injury in Chelsea’s FA Cup third-round replay with Norwich next Wednesday.
Provisional squad: Courtois, Caballero, Rudiger, Alonso, Fabregas, Drinkwater, Kante, Hazard, Morata, Pedro, Bakayoko, Moses, Kenedy, Musonda, Zappacosta, Willian, Batshuayi, Cahill, Christensen, Azpilicueta, Luiz, Clarke-Salter, Sterling, Eduardo, Ampadu, Hudson-Odoi.
Colchester will hand late fitness tests to Ryan Jackson and Brandon Comley prior to their clash with Yeovil.
Arsenal secured their place in the quarter-finals of the Europa League following their 3-1 defeat of AC Milan at the Emirates Stadium on Thursday.
Danny Welbeck scored twice against AC Milan to toast his England recall by sending Arsenal into the last eight of the Europa League.
Ipswich striker Joe Garner will not play again this season after it emerged he suffered a fractured skull against Sheffield United last week.