The 30-year-old suffered ligament damage in his back that ruled him out of last week’s 2-2 draw with his former club Manchester United, and he will not be risked for the trip to take on Rafa Benitez’s men.
Hughes will also consider the fact that the Potters face Chelsea next weekend without Kurt Zouma, who is ineligible against his parent club as he is on loan at Stoke.
“It’s touch and go,” Hughes said. “In fairness, the last few weeks prior to the injury during the international week we tried to manage Ryan’s workload and then he came back and got injured in training.”
“We will check him again today and he may come in with a really positive attitude towards the game at the weekend and then I have a decision, but as things stand, it’s unlikely.”
Elsewhere, Geoff Cameron is the only other short-term injury worry after he picked up a hamstring problem in the first half of last week’s game with United.
Sheffield United could have leading scorer Leon Clarke back for their home game against Nottingham Forest.
St Johnstone midfielder Murray Davidson is determined to use their derby exploits as a springboard to rid them of inconsistency.
Lincoln will once again be without skipper Luke Waterfall for Saturday's League Two derby clash with Grimsby.
Leeds boss Paul Heckingbottom is expected to choose from an unchanged squad for the home game against Sheffield Wednesday.
Derek Fazackerley will take the Oxford helm on the 56th day of his caretaker stint in Saturday's match against Peterborough.