Dixon has a hip problem, Ashe is suffering with a knee injury and Eastmond has a groin strain following Saints' cup victory.
Coach Royce Simmons confirmed the blows and the trio are all doubtful to face high-flying Huddersfield in the Super League next weekend.
Simmons told the St Helens Star: "Dixon got a bruised hip and could not go back on.
"Nathan Ashe already had a knee problem but played with it and then Kyle Eastmond suffered a groin strain late on and had to be brought off.
"The doc is working on them now."