The sides will square off in Naples with Inter sitting in eighth place in the Italian top-flight standings, one place but four points behind Napoli.
However, the Nerazzurri will enter the contest on a decent run of form, having taken 10 points from their last five matches.
The Partenopei have claimed eight points from a possible 15 and it has all the makings of a tight encounter on Friday evening, but Pioli has made it clear his men will not take a backward step against their rivals.
“We have done well so far and through our daily work I believe we can improve even more,” he said. “We certainly have no fear about the match tomorrow because each game has its own dangers. We have big personalities in this team and that’s important.”
A goal apiece from James Tomkins and Luka Milivojevic handed Crystal Palace a crucial 2-0 win over Huddersfield at the John Smith's Stadium on Saturday evening.
Manchester United bounced back from their Champions League exit as they beat Brighton 2-0 at Old Trafford on Saturday night to reach the FA Cup semi-finals.
Tottenham reached the FA Cup semi-finals after they cruised to a 3-0 victory over Swansea in their quarter-final clash at the Liberty Stadium on Saturday.
Premier League basement club West Brom squandered the lead as they suffered a 2-1 defeat to Bournemouth at the Vitality Stadium on Saturday.
Chesterfield boss Jack Lester felt his side were unlucky not to claim all three points after their 1-1 draw at Cheltenham.
New Swindon manager Phil Brown revelled in his side's victory at Cambridge in his first game in charge.