Tottenham travel to Leverkusen on Tuesday and then host Bayer at Wembley in their next Champions League fixture on Wednesday, November 2.
Spurs sit second in Group E with three points from two games having won at CSKA Moscow after opening the tournament with a home defeat to Monaco.
Bayer have two points from as many games and Pochettino has urged his side to target a double over the German club as they push for a place in the knockout stages.
"It's important, we must win," he said. "If we want to go to the next round, this is key to take three points from this game and to play at Wembley the same way.
"The next two games against Leverkusen for us hold the key to the possibility of going to the next round."